Methyl Primer Express Software is a specialized primer design tool for epigenetics research that streamlines assay development on bisulfite‑converted DNA. It supports methylation‑specific PCR (MSP) and bisulfite sequencing by identifying CpG sites within user‑provided sequences and proposing primer pairs that either discriminate between methylated and unmethylated templates or amplify regions regardless of methylation status.
The software accounts for bisulfite conversion rules, helps avoid CpG dinucleotides in primer binding regions when appropriate, and reports key design metrics such as amplicon length, melting temperature (Tm), and GC content. An intuitive interface highlights CpG density and primer locations, making it easier to target promoters, CpG islands, and other regions of interest. By automating primer selection for bisulfite‑modified DNA, Methyl Primer Express helps researchers rapidly develop robust assays for DNA methylation studies, from basic research to biomarker validation.
Methyl Primer Express Software is developed by casa. The most popular version of this product among our users is 1.0. The name of the program executable file is Methyl Primer Express v1.0.exe.
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